{"id":2384,"date":"2024-12-23T15:19:55","date_gmt":"2024-12-23T07:19:55","guid":{"rendered":"https:\/\/sunshineivf.com.tw\/?p=2384"},"modified":"2025-06-12T15:20:35","modified_gmt":"2025-06-12T07:20:35","slug":"hello-world-76","status":"publish","type":"post","link":"https:\/\/sunshineivf.com.tw\/zh\/hello-world-76\/","title":{"rendered":"\u9451\u5b9a\u7cbe\u5b50\u7f3a\u9677\u776a\u4e38\u5207\u7247\u4e2d\u7684\u5dee\u7570\u8868\u9054\u57fa\u56e0"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"2384\" class=\"elementor elementor-2384\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5f5e48 e-flex e-con-boxed e-con e-parent\" data-id=\"5f5e48\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-714db014 elementor-widget elementor-widget-text-editor\" data-id=\"714db014\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div>\u9451\u5b9a\u7cbe\u5b50\u7f3a\u9677\u776a\u4e38\u5207\u7247\u4e2d\u7684\u5dee\u7570\u8868\u9054\u57fa\u56e0<\/div>\n<div>\u00a0<\/div>\n<div><a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/rmb2.12616\">https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/rmb2.12616<\/a><\/div>\n<div>\u00a0<\/div>\n<div>\u900f\u904e RNA \u5b9a\u5e8f (RNA-Seq) \u91cf\u5316\u8f49\u9304\u7570\u5e38\u7cbe\u5b50\u7684\u776a\u4e38\u7d44\u7e54\u4e2d\u5dee\u7570\u8868\u9054\u7684\u8f49\u9304\u672c\uff0c\u5f9e\u800c\u63ed\u793a\u904e\u6ffe\u5dee\u7570\u8868\u9054\u57fa\u56e0\uff08DEG\uff09 \u900f\u904e qRT-PCR \u9a57\u8b49\u4e86\u9078\u5b9a\u7684 10 \u500b DEG\uff0c\u4e26\u5728\u776a\u4e38\u6d3b\u6aa2\u4e2d\u78ba\u5b9a\u4e86\u5169\u7a2e\u86cb\u767d\u8cea\u7684\u5b9a\u4f4d\u3002\u627e\u51fa\u8abf\u7bc0\u7cbe\u5b50\u5f62\u614b\u548c\u529f\u80fd\u7684\u65b0\u57fa\u56e0\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u5206\u6790\u63ed\u793a 6 \u500b\u57fa\u56e0\uff08SPATA31E1\u3001TEKT3\u3001SLC9C1\u3001PDE4A\u3001CFAP47 \u548c TNC\uff09\u8207\u4eba\u985e\u7cbe\u5b50\u767c\u80b2\u9ad8\u5ea6\u76f8\u95dc<\/div>\n<div>\u00a0<\/div>\n<div>\u776a\u4e38\u5207\u7247\u5169\u7a2e\u86cb\u767d ORAI1 \u548c SPATA31E1 \u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5b9a\u4f4d\u8b49\u5be6\u5728\u767c\u80b2\u4e2d\u7684\u4eba\u985e\u751f\u6b96\u7d30\u80de\u4e2d\u8868\u9054\uff0c\u5176\u4e2d SPATA31E1 \u5728\u665a\u671f\u7cbe\u6bcd\u7d30\u80de\u548c\u7cbe\u5b50\u7d30\u80de\u8868\u73fe\u660e\u986f<\/div>\n<div>\u00a0<\/div>\n<div>\u6b64\u7814\u7a76\u65b9\u6cd5\u53ef\u627e\u51fa\u4e86\u5f71\u97ff\u4eba\u985e\u751f\u6b96\u7d30\u80de\u7684\u57fa\u56e0\u53ca\u76f8\u95dc\u86cb\u767d\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>Identification of differentially expressed genes in human testis biopsies with defective spermatogenesis<\/div>\n<div>Methods<\/div>\n<div>Human testis biopsies were collected from men with well-characterized phenotypes of normal spermatogenesis, spermatid arrest, and Sertoli cell-onlyphenotype, and transcriptional differences were quantified by RNA-sequencing (RNA-Seq). Differentially expressed genes (DEGs) were filtered based on predominant expression in spermatids and gene functional annotations relevant to spermmorphology and motility. Selected 10 DEGs were validated by qRT-PCR and the localization of two proteins was determined in testis biopsies.<\/div>\n<div>\u00a0<\/div>\n<div>Results<\/div>\n<div>The analysis revealed 6 genes (SPATA31E1, TEKT3, SLC9C1, PDE4A, CFAP47, and TNC) that are excellent candidates for novel genes enriched in developinghuman sperm. The immunohistochemical localization of two proteins, ORAI1 and SPATA31E1, in testis biopsies, verified that both are expressed indeveloping human germ cells, with SPATA31E1 enriched in late spermatocytes and spermatids.<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u9451\u5b9a\u7cbe\u5b50\u7f3a\u9677\u776a\u4e38\u5207\u7247\u4e2d\u7684\u5dee\u7570\u8868\u9054\u57fa\u56e0 \u00a0 https:\/\/onlinelibrary.wiley.com\/do [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1952,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[16,12],"tags":[],"class_list":["post-2384","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-16","category-12"],"_links":{"self":[{"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/posts\/2384","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/comments?post=2384"}],"version-history":[{"count":4,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/posts\/2384\/revisions"}],"predecessor-version":[{"id":2388,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/posts\/2384\/revisions\/2388"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/media\/1952"}],"wp:attachment":[{"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/media?parent=2384"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/categories?post=2384"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sunshineivf.com.tw\/zh\/wp-json\/wp\/v2\/tags?post=2384"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}