Conventional thawing temperature is set at 37 degrees Celsius. This article points out that increasing the thawing temperature of spermatozoa to 42 degrees Celsius will result in higher spermatozoa survival motility.
Progressive motility of sperm warmed at 38, 40 and 42 °C was 26.4 ± 8.4%; 56.6 ± 16.3% and 65.4 ± 15%
Andrologia
. 2016 Feb;48(1):111-3. doi: 10.1111/and.12406. Epub 2015 Feb 3.
High temperature is essential for preserved human sperm function during the devitrification process
Sperm vitrification is a cryopreservation method based on high-speed freezing by direct exposure of cells in liquid nitrogen (N2L), thereby avoiding the traditional cooling curves of freezing. The objective of this work was to determine the optimal warming temperature for vitrified human spermatozoa in order to maintain their fertilisation potential. The objective of this work was to determine the optimal warming temperature for vitrified human spermatozoa in order to maintain their fertilisation potential. Spermatozoa were cryopreserved by direct plunging into N2L and warmed at different temperatures Sperm motility was evaluated by the CASA system and the sperm membrane function by HOST test. It was detected that progressive motility of sperm warmed at 38, 40 and 42 °C was 26.4 ± 8.4%; 56.6 ± 16.3% and 65.4 ± 15%, respectively, with statistically significant differences between the temperatures of 38, 40 and 42 °C. The sperm membrane function was evaluated by the CASA system and the sperm membrane function by HOST test. significant differences between the temperatures of 38 and 40 °C and 38 and 42 °C (P < 0.05). The plasma membrane function evaluated by HOST test was better preserved at 42 °C (76.3 ± 2.0%) compared to 40 °C (43 ± 2%) and 38 °C (65.6 ± 1.5%). . The temperature in the thawing process can affect the motility and plasma membrane integrity and function. The warming at 42 °C for thawed vitrified sperm is the optimum temperature to preserve the sperm's ability to survive. The warming at 42 °C for thawed vitrified sperm is the optimum temperature to preserve the sperm physiological parameters.