冷凍方式採用傳統甘油glycerol液態氮蒸氣2段式冷凍

 

Endocrinol Diabetes Metab Case Rep

. 2023 Apr 12;2023(2):22-0402. doi: 10.1530/EDM-22-0402. Print 2023 Apr 1.

Live birth using autologous sperm cryopreserved for 26 years

Summary: We report the successful delivery of a healthy baby after intracytoplasmic sperm injection (ICSI) with frozen-thawed autologous sperm,cryostored for 26 years, the longest successful autologous sperm cryostorage reported. Sperm was cryostored for a 15-year-old boy at the time of hiscancer diagnosis. Semen samples were frozen with cryoprotectant, using a graduated vapour-phase nitrogen protocol. Straws were stored in a largevapour-phase nitrogen tank until transfer for use. The couple underwent a single ICSI-in vitro fertilisation procedure using the frozen-thawed spermwith a transfer of five fertilised embryos, resulting in the live birth of a healthy baby boy. This reinforces the importance of offering spermcryopreservation to men who have not completed their family prior to gonadotoxic treatment for cancer or other diseases. As practical, low-costfertility insurance, it should be offered to any young man who can collect semen and it provides essentially unlimited duration of fertilitypreservation.