Identification of Differently Expressed Genes in Sperm Defective Testis Sections

 

 

Quantification of differentially expressed transcripts in the testicular tissue of transcribed abnormal spermatozoa by RNA sequencing (RNA-Seq) to reveal filtered differentially expressed genes (DEGs) Ten selected DEGs were verified by qRT-PCR and the localization of two proteins was determined in testicular biopsies. New genes regulating sperm morphology and function were identified.

 

Analysis reveals 6 genes (SPATA31E1, TEKT3, SLC9C1, PDE4A, CFAP47 and TNC) highly associated with human spermatogenesis

 

Immunohistochemical localization of two proteins, ORAI1 and SPATA31E1, in testis sections confirmed their expression in developing human germ cells, with SPATA31E1 being significantly expressed in advanced spermatogonia and spermatocytes.

 

This research method identifies genes and related proteins that affect human germ cells. 

 

 

 

Identification of differentially expressed genes in human testis biopsies with defective spermatogenesis

Methods

Human testis biopsies were collected from men with well-characterized phenotypes of normal spermatogenesis, spermatid arrest, and Sertoli cell-only phenotype, and transcriptional differences were quantified by RNA-sequencing (RNA-Seq). Differentially expressed genes (DEGs) were filtered based on predominant expression in spermatids and gene functional annotations relevant to sperm morphology and motility. Differentially expressed genes (DEGs) were filtered based on predominant expression in spermatids and gene functional annotations relevant to sperm morphology and motility. Selected 10 DEGs were validated by qRT-PCR and the localization of two proteins was determined in testis biopsies.

 

Results

The analysis revealed 6 genes (SPATA31E1, TEKT3, SLC9C1, PDE4A, CFAP47, and TNC) that are excellent candidates for novel genes enriched in developing The immunohistochemical localization of two proteins, ORAI1 and SPATA31E1, in testis biopsies, verified that both are expressed in developing human germ cells, with SPATA31E1 being the most important. developing human germ cells, with SPATA31E1 enriched in late spermatocytes and spermatids.