The technique of using density gradient centrifugation to separate the sex of dog sperm is not effective.

 

Anim Reprod Sci

. 2018 Dec:199:84-92.  

Sperm sexing with density gradient centrifugation in dogs

 Sperms were separated using gradients of Percoll® or Ficoll. 

The percentage of sperm containing X and Y chromosomes was evaluated by qPCR. 

The use of the Ficoll gradient resulted in the greatest sperm quality after centrifugation. 

No sperm enhancement containing X or Y chromosome occurred with use of any of the methods 

Percoll® 54.8 ± 1.9 compared with 45.2 ± 1.9. 

Percoll® associated with Nycodenz® 53.2 ± 2.0 compared with 46.8 ± 2.0. 

Ficoll 55.0 ± 1.5 compared with 45.0 ± 1.5 

for the percentages of cells containing the X and Y chromosomes, respectively

The technique of sexing dog sperm using density gradients was not effective for commercial application.