The Ficoll-400 Density Gradient can be used to separate spermatozoa & produce a higher percentage of live, mature, morphologically normal spermatozoa.

 

 

 

Layering: 5% (v/v) density top layer and 90% (v/v) Ficoll-400 density lower layer. 

 

 

Density comparison: Ficoll density 1.2 g/ml

 

--normal spermatozoa (1.17 g/ml)

 

--immotile immature sperm cells having lower density of 1.02 g/ml.

 

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Ficoll-400 density gradient method as an effective sperm preparation technique for assisted reproductive techniques J Hum Reprod Sci. 2016 Jul-Sep; 9( 3): 194-199.

 

The Ficoll-400 density gradient yielded higher percentage of live, mature, morphologically normal spermatozoa in an isolated fraction as compared to the other three techniques. Ficoll-400 density gradient yielded higher percentage of live, mature, morphologically normal spermatozoa in an isolated fraction as compared to